《SNIA-SDC23-Wisinewski-Verona-Bit-to-DNA-Writing-Machines_1.pdf》由会员分享,可在线阅读,更多相关《SNIA-SDC23-Wisinewski-Verona-Bit-to-DNA-Writing-Machines_1.pdf(25页珍藏版)》请在三个皮匠报告上搜索。
1、1|2023 SNIA.All Rights Reserved.Virtual ConferenceSeptember 28-29,2021Bit-to-DNA Writing Machinesa Microfluidic Platform and Future Data Center Operation OverviewHenrique Reis WisinewskiBruno Marinaro Veronahenriquereisipt.br;brunoveronaipt.br2|2023 SNIA.All Rights Reserved.About usSince 2021,the Pr
2、ometheus group has been working on converting DNA synthesis from Biotech industry into Data Storage applicationWe are a multidisciplinary group that develop technology for many aspects of the molecular data pipeline,such as biology,biochemistry,computation and microfabrication3|2023 SNIA.All Rights
3、Reserved.The issue at handCredits:IDCs Data Age 2025:The Evolution of Data to Life Critical,study163ZB 30%being critical or hypercritical data 60%being Cold Data4|2023 SNIA.All Rights Reserved.The issue at handCredits:Gartner Market Trends:Evolving Enterprise Data Requirements-How much is Not Enough
4、?apud:Preserving our Legacy:An Introduction to DNA Data Storage,by DNA Data Storage Alliance Traditional forms of Data Storage may not be enough5|2023 SNIA.All Rights Reserved.Artificial DNA Synthesis 101ATCCCouplingOxidationDeblocking6|2023 SNIA.All Rights Reserved.Where it all began First ever art
5、ificial DNA synthesizer machine 15 bases per day(max of 30 bits per day)“The Coder Model 280”DNA synthesizer,released in 1980 Credits:Vega Biotechnologies7|2023 SNIA.All Rights Reserved.Current standard machines for Biotech Tabletop equipment Essencially the same design concept as before,justoptimiz
6、ed 768 oligos per run In 24 hrs,it can build strandsof 148 nt Estimate of 28 kB in a day2.6 bits/sec“Dr.Oligo 768XLc”DNA synthesizer,for biotech applicationsCredits:BiolyticLab Performance,Inc.8|2023 SNIA.All Rights Reserved.Examples of innovative new devicesShannon,CATALOGs prototype DNA writer Cre
7、dits:CATALOGTwist Biosciences CMOS-based chip for DNA synthesis Credits:Twist BioscienceIPT/Lenovos LTCC-based chips for DNA synthesisCustomArrays CMOS-based chip for DNA synthesis Credits:CustomArray Inc.9|2023 SNIA.All Rights Reserved.No matter how,its a fluidic process all the same“Macrofluidic”M
8、icrofluidic10|2023 SNIA.All Rights Reserved.Microfluidic TechnologyMicrofluidic technology:Manipulation and control of fluids within environments on a scale of micrometers Smaller footprints Reduced reagent volumes Safer system More sustainable operationMicrofluidic chemostatScience,v.309,n.5731,200
9、5.10.1126/science.1109173apud:Nature,v.442,n.7101,2006.doi:10.1038/nature0505811|2023 SNIA.All Rights Reserved.Microfluidic TechnologyMicrofluidic technology:Manipulation and control of fluids within environments on a scale of micrometersIlluminas Flow Cell:Next Seq 500Credits:Illumina Inc.Droplet g
10、eneratorCredits:Dolomite 12|2023 SNIA.All Rights Reserved.Microfluidic Technology Is microfluidic suitable for industrial applications?Microfluidic chipResearch levelFull/Industrial-scale prototypeDeployment levelLab-scale prototypeDevelopment level13|2023 SNIA.All Rights Reserved.Microfluidic Techn
11、ology Is microfluidic suitable for DNA synthesis?“Dr.Oligo 768XLc”DNA synthesizer,for biotech applicationsCredits:BiolyticLab Performance,Inc.IPT/Lenovos LTCC-based chips for DNA synthesis PoCIPT/Lenovo Ceramic“DNA writer”Device:1.1 cm(0.67 in)Well:6 mm14|2023 SNIA.All Rights Reserved.Microfluidic T
12、echnology Is microfluidic suitable for DNA synthesis applied to Data Storage?15|2023 SNIA.All Rights Reserved.How will the microfluidic technology enable the development of bit-to-DNA writing machines?Microfluidic Technology 750 L .075 L Mass parallelization!16|2023 SNIA.All Rights Reserved.How will
13、 the microfluidic technology enable the development of bit-to-DNA writing machines?Microfluidic Technology 750 L .075 L Mass parallelization!17|2023 SNIA.All Rights Reserved.Pushing further the TRL of the bit-to-DNA writing machines How far are we from a commercially viable Bit-to-DNA writing machin
14、e?1Basic principles observed2Technology concept formulated3Experimental proof of concept4Technology validated in lab5Technology validated in simulated simulated environmentenvironment6Technology demonstrated in simulated simulated environmentenvironment7System model/prototype demonstration in operat
15、ional environment8System complete and qualified9Actual system proven in operational environmentResearchDevelopmentDeployment*TRL=Technology Readiness Level18|2023 SNIA.All Rights Reserved.Pushing further the TRL of the bit-to-DNA writing machines Are the bit-to-DNA writing machines ready for deploym
16、ent into real data centers?Meet data center requirements:Compatible writing throughput;Small footprint;Simple and low cost data center facility retrofitting;No need for high specialized personnel;Low complexity and low cost reagents handling;Environment conditions similar to the current data center
17、facilities.LTO Tapes(18TB)400 Mb/sec 220 cm(13,4 in)For reference:Dr.Oligo 768 wells 2.6 b/sec 422000 cm(25750 in)19|2023 SNIA.All Rights Reserved.Pushing further the TRL of the bit-to-DNA writing machines Pushing the maturity level to TRL 5 and beyond:Focus on the outer parts/surrounding of the chi
18、ps20|2023 SNIA.All Rights Reserved.Pushing further the TRL of the bit-to-DNA writing machines Pushing the maturity level to TRL 5 and beyond:Credits:French National Archives,AE/II/2983 and AE/II/2982Apud:CNRS News websiteCredits:Stphane Lemaire,from CNRS Sorbonne UniversitApud:CNRS News website Froz
19、en Storage is the perfect target client for first level DNA Data Storage systems21|2023 SNIA.All Rights Reserved.Pushing further the TRL of the bit-to-DNA writing machines Pushing the maturity level to TRL 5 and beyond:Parallelize existing devices instead of proposing new chip designs+First generati
20、on of writing machines.Multiple microfluidic chips22|2023 SNIA.All Rights Reserved.Pushing further the TRL of the bit-to-DNA writing machines How far are we from a commercially viable Bit-to-DNA writing machine?1Basic principles observed2Technology concept formulated3Experimental proof of concept4Te
21、chnology validated in lab5Technology validated in simulated simulated environmentenvironment6Technology demonstrated in simulated simulated environmentenvironment7System model/prototype demonstration in operational environment8System complete and qualified9Actual system proven in operational environ
22、mentResearchDevelopmentDeployment23|2023 SNIA.All Rights Reserved.Closing thoughts Microfluidic technology is one of pillars to make commercially viable bit-to-DNA writing machines Massive parallelization will lead to miniaturized manipulation of fluids Microfluidic can be used to produce commercial
23、ly viable products Promising microfluidic chips proposed by many groups There is still room for improvements for higher yields and throughput(to explore the full potential of DNA Data Storage)24|2023 SNIA.All Rights Reserved.Technology status:academia(research TRLs)and industry(deployment TRLs)are c
24、loser Next step to bridging this gap:focus on the surrounding of the microfluidics chips and start working with real data storage routinesClosing thoughtsTechnological“valley of death”Credits:Alessandro Rossini,Bridging the technological“valley of death”,PwC Norway25|2023 SNIA.All Rights Reserved.Please take a moment to rate this session.Your feedback is important to us.