1、单单一氨氨基酸对对于植物生长长的影响响 Effect of Amino Acids As Bio-stimulant 2019.05.172019.05.17 Whenever,Wherever,Whatever Whenever,Wherever,Whatever You You EatEat,CJ,CJ BIO is There BIO is There!Contents 1 1.简简介介 IntroductionIntroduction 2.2.生物刺激生物刺激剂剂 BioBio-StimulantStimulant 3 3.植物植物营养营养 氨氨基酸基酸 Plant nutrition

2、 Amino acids Plant nutrition Amino acids 4 4.研研究方法和究方法和实验实验 R&D Strategy&Trial R&D Strategy&Trial *KIAKIA 希杰生化集团介绍 CJ CheilJedang Bio Introduction 生化事业开启MSG77 核苷酸销售 生物研发中心建立 全球事业 赖氨酸 苏氨酸 色氨酸研究院 精氨酸缬氨酸2018 销售额Sales Revenue$5.0 bil 销售销售分公司及工厂厂分布 Sales office and Plant location America R&D Post Shangha

3、i Hochiminh Pasuruan Plant Moscow India Tokyo Osaka Taiwan Philippines Shenyang Plant 沈阳工厂 Bangkok Liaocheng Plant 聊城工厂 Jombang Plant Guangzhou Malaysia Plant Frankfurt Shenyang Jinan Beijing London France Jordan Jakarta Nigeria Kenya Kuala lumpur Tianjin Sichuan HQ R&D Post 生产工厂Production plant：8个（

4、Post）销售分公司Sales office：27个（Office）研发中心 R&D Post America Plant Chicago Brazil Plant Sao Paulo New Jersey Colombia Chile Ningbo Plant 宁波工厂 提高对非生物胁迫的耐受性提高对非生物胁迫的耐受性 Improve tolerance to AbioticAbiotic stressstress 1 提高养分利用率提高养分利用率 Improve nutrients USE EfficiencyUSE Efficiency 2 提高作物品质提高作物品质 Improve Cr

5、op quantityCrop quantity 3 促进养分吸收促进养分吸收 Improve nutrients UptakeUptake 4 生物刺激剂可提高农业效率 BioBio-Stimulants increase agricultural efficiencyStimulants increase agricultural efficiency 生物刺激剂剂 Bio-stimulant 微生物菌剂 Microbial inoculants 腐植酸/黄腐酸 Humic acids/Fulvic acids 蛋白水解物及氨基酸 Protein hydrolysates and amin

6、o acids 海藻提取物 Seaweed extracts 酸/碱水解（L,D-型氨基酸）Acid/Alkali hydrolysis (L,D-form amino acid)微生物发酵（L-型氨基酸）Microbial fermentation (L-form amino acid)类别类别 TypeType 功效功效 FunctionFunction 什么么是氨氨基酸？What are Amino-acids?由一个氨基酸(NH2),1个羧基(COOH),1个R基团（或侧链）组成的有机物，每个氨基酸有不同的R基团 A group of organic molecules that co

7、nsist of a basic amino group(NH2),an acidic carboxyl group(COOH),and an organic R group(or side chain)that is unique to each amino acid 氨基酸是地球上所有生物体的基本组成部分 Amino acids are the basic building blocks of all life on earth 维持生命所需基本氨基酸为20种 20 standard amino acids needed for life 氨基酸，除甘氨酸外，基本都存在两种异构体形式，D型

8、及L-型 Amino acids,except glycine,has two forms named D(meaning of right)and L(meaning of left).只有只有L L-型具有生物活性型具有生物活性 Only the L-form is biologically active 大多数植物都有可合成所需的所有氨基酸的能力：但该过程需消耗能量：最好可通过叶面或根系来提供可直接使用的L-氨基酸 Most plant have ability to synthesize all the amino acids needed,but energy intensive p

9、rocess:its a good idea to provide Ready to use L-amino acids via foliar or root feeding.L L-型型 VS DVS D-型型氨氨基酸基酸 L L-form VS Dform VS D-form Amino acids form Amino acids 氨氨基酸基酸结构结构 Structure of Amino acidsStructure of Amino acids 氢键 羧基 氨基 R基团 水解法 VS 发发酵法 Hydrolysis Vs.Fermentation 不同于目前不同于目前大多大多数氨数氨

10、基酸肥料基酸肥料，其中的其中的氨氨基酸基本都是通基酸基本都是通过过强酸或强强酸或强碱碱水解水解获获取取，CJCJ的的氨氨基酸均是采用生物基酸均是采用生物发发酵工酵工艺获艺获取的取的。Unlike the most amino acid fertilizers which are obtained by acid/alkali hydrolysis,CJ Amino acids are made by microbial fermentation process 植物来源(甘蔗、木薯、玉米等.)Vegetal Origin(Sugar Cane,corn,Tapioca etc.)1 2 微生物

11、菌种 Microorganism 3 发酵 Fermentation L L-型型氨氨基酸基酸 L L-form Amino acidsform Amino acids 4 4 生物生物发发酵酵 Microbial fermentationMicrobial fermentation 动物来源(羽毛、毛发等.)Animal Origin (Feather meal,Gelatin etc.)1 2 H2SO4,HCl,NaOH etc.3 水解分离获取单一氨基酸 Hydrolyzation Breakdown into AA DLDL-型型氨氨基酸基酸 DLDL-form Amino acid

12、sform Amino acids 4 4 化化学学水解水解 Strong chemical HydrolysisStrong chemical Hydrolysis 粉&液 Powder&Liquid 饲料级&食品级 Feed&Food grade CJ生物发发酵技术术 Technology of CJ Cheiljedang Bio 微生物发酵 车间 Microbe Factory 微生物 Microbe 培养 Assimilated 发酵 Fermentation 甘蔗甘蔗 Sugar CaneSugar Cane 木薯木薯 TapiocaTapioca 玉米玉米 CornCorn 仅仅

13、通通过过生物生物发发酵工酵工艺艺生生产产 Only through a biological fermentation processOnly through a biological fermentation process 生物生物发酵原料发酵原料 BIO MaterialBIO Material 生物发酵过程生物发酵过程 BIO ProcessBIO Process 生物发酵产品生物发酵产品 BIO ProductBIO Product CJ 氨氨基酸 CJ Amino Acids 通通过过生物生物发发酵工酵工艺艺生生产产的植物的植物来来源的源的L L-型型氨氨基酸基酸 Vegetabl

14、e origin LVegetable origin L-form amino acids produced by microbial fermentation processform amino acids produced by microbial fermentation process 纯纯度：度：98%98%，100%100%具有生物活性的具有生物活性的L L-型型氨氨基酸基酸 Purity 98%(min.),100%of bioactive LPurity 98%(min.),100%of bioactive L-form amino acidsform amino acids

15、:可根据每种氨基酸的功能设计制定配方 Able to make formulation based on each amino acids function 植物植物营养营养用用CJ CJ 氨氨基酸基酸 CJ CJ Amino acids specification for Plant Amino acids specification for Plant nutritionnutrition 分子式 含量 溶解性 粗蛋白 感官 堆密度 分子量 精氨酸 色氨酸 赖氨盐酸盐 苏氨酸 缬氨酸 蛋氨酸 核苷酸 浅黄色粉末 白色或淡褐色色粉末 白色结晶性粉末 白色结晶性粉末 白色粉末 研究方法及实验

16、R&D Strategy&Trial 1.1.植物植物营养研营养研究方法究方法 Plant Nutrition R&D Strategy Plant Nutrition R&D Strategy 2.2.基于基于转录组学研转录组学研究究L L-型型氨氨基酸基酸对对于植物的作用模式于植物的作用模式 The mode of action of LThe mode of action of L-amino acid based on amino acid based on TranscriptomicsTranscriptomics 3.3.CJCJ氨氨基酸基酸对对作物生作物生长长的影的影响响 Ef

17、fects of CJ amino acids on growth of crops Effects of CJ amino acids on growth of crops 植物营养研营养研究方法 Plant Nutrition R&D Strategy 1 1 确确定每定每种单体氨种单体氨基酸的作用模式基酸的作用模式 Identifying mode of actions of each single Amino acidsIdentifying mode of actions of each single Amino acids 转录组学转录组学：识别实验植物中氨基酸的作用模式MoA，拟

18、南芥 TRANSCRIPTOMICS:TRANSCRIPTOMICS:Identify the MoA of AAs in the model plants,Arabidopsis 2 2 客客户户定制化配方定制化配方设计设计&功效功效实验实验 Customized Customized recipe design&Efficacy recipe design&Efficacy testtest 表型表型组学组学（温温室室实验实验 PHENOMICSPHENOMICS (Lab&Green hous(Lab&Green house e)对于温室实验作物使用氨基酸生物刺激剂的功效实验 Effic

19、acy test of AA-biostimulant with target crop plants in the glass house 大田大田实验实验 FIELD TRIALSFIELD TRIALS 对大田作物进行氨基酸生物刺激剂的功效实验 Efficacy test of AA-biostimulant with target crop plants in the field 配方配方设计设计 Design recipeDesign recipe 针对特定需求提供定制化配方设计 如抗旱、色泽等 Customized recipe design for specific purpos

20、e (e.g.drought,coloring etc.,)Pathway analysisPathway analysis 1Gene Ontology(GO)term enrichment is a technique for interpreting sets of genes making use of the Gene Ontology system of classification,in which genes are assigned to a set of predefined bins depending on their functional characteristic

21、s 转录组学转录组学：研研究植物作用模式 The mode of action of L-amino acid based on Transcriptomics 1.1.表型分析表型分析 Phenotype analysisPhenotype analysis 转录组学：分析使用氨基酸前后基因表达变化 TranscriptomicsTranscriptomics :analyze changes of gene expression by Amino Acids treatment 2.2.基因分析基因分析 Microarray analysisMicroarray analysis 分析22

22、,810拟南芥基因表达 Analyze the expression of 22,810 Arabidopsis genes 植物叶片喷施单体氨基酸植物叶片喷施单体氨基酸 Spray single AA on plant leavesSpray single AA on plant leaves Amino acid 氨基酸氨基酸 Related mechanism 相关机理相关机理 A 激素(CK,BR,ER)，信号转导 Hormone signal transduction 生长素稳定 Auxin homeostasis B 激素CK信号转导 Hormone(CK)signal trans

23、duction 生长素稳定 Auxin homeostasis 乙烯生物合成 Ethylene biosynthesis C 生长素信号转导与平衡 Auxin signal transduction&homeostasis 光合作用 Photosynthesis DNA复制与修复 DNA replication&repair 细胞壁及表皮发育 Cell wall&cuticle development D 细胞壁及表皮发育 Cell wall&cuticle development 授粉 Pollination 激素CK,ER 信号转导 Hormone(CK,ER)signal transdu

24、ction E 系统获得抗性 Systemic acquired resistance(SAR)抗氧化能力 Resistance to oxidative stress 茉莉酸新陈代谢 Jasmonic acid metabolism 花青素生物合成 Anthocyanin biosynthesis F 生物合成及信号转导 SA biosynthesis&signal transduction SA 抗病性 Pathogen resistance 抗氧化能力 Resistance to oxidative stress 激素稳定 Hormone homeostasis G 生长素稳定 Auxi

25、n signal transduction 激素生物合成ABA,生长素,BR Hormone biosynthesis(ABA,auxin)葡糖基转移酶 UDP-glucosyl transferase 解毒 Detoxification H 促进生长 Growth promotion 解毒 Detoxification 抗生物及非生物胁迫 Resistance to biotic&abiotic stress 表型分析表型分析 Phenotype analysis Phenotype analysis CK,细胞分裂素Cytokinin;ER,乙烯Ethylene;BR,芸苔素甾醇Brasi

26、nosteroid;JA,茉莉酸Jasmonic acid SAR,系统获得抗性Systemic Acquired resistance;SA,水杨酸Salicylic acid L-氨氨基酸应应用于作物实验实验 L-amino acids Crop test 内内容容 ContentsContents 作物作物 CropCrop 移栽日期移栽日期 Date Date ofof TransplantingTransplanting FertilizationFertilization (g/mg/m2 2)应应用用 Application Application of amino of ami

27、no acidsacids 观观察察Growth checkGrowth check 检测检测指指标标 ParametersParameters 基肥基肥 BasalBasal 追肥追肥 AdditionalAdditional 频频率率 FrequencyFrequency 浓浓度度 Conc.Conc.日期日期 DateDate 频频率率 FrequencyFrequency 日期日期 DateDate 叶类 Leaf Vegetable 生菜 Lettuce 6.24 N:10.0 P2O5:5.9 K2O:6.4 N:10.0 P2O5:0 K2O:6.4 2 -0.2%(w/)氨基酸

28、水溶液AA soln -50mL/株plant -喷施 Foliar application 7.04 7.14 1 7.25 -地上部重量（鲜重、干重）Shoot weight(fresh&dry)-叶面积 Leag Area -叶绿素相对含量SPAD 小白菜 PakChoi 6.24 果蔬 Fruit Vegetable 西红柿 Tomato 7.7 N:13.6 P2O5:16.4 K2O:7.9 N:10.4 P2O5:0 K2O:15.9 6 -0.2%(w/)AA soln -100mL/株plant -喷施 Foliar application *追肥：0.2%蛋氨酸溶液 (10

29、0mL/株plant)Additionally,0.2%methionine soln was applied to growth bed (100 mL/plant)7.17 7.31 8.14 8.27 9.10 9.24 5 8.30 9.6 9.13 9.28 10.23 -果实数 Fruit Qty、重量 weight、含糖量 Brix -株高 Height,茎粗 Stem diameter,SPAD,茎重（鲜重、干重、10.23）shoot weight (Fresh&dry,23rd Oct)果树 Fruit tree 苹果 Apple N/A 常规管理 Conventional

30、 Management 4 -0.2%(w/)氨基酸水溶液AA soln -4L/棵tree -喷施 Foliar application 7.13 8.11 9.9 10.7 3 10.24 10.31 11.17 -果实重量 Fruit weight,含糖量 Brix,着色Colorization*注：生菜、小白菜、番茄的施肥量是根据韩国农村发展局要求；所有作物实验均进行3次重复实验，生菜进行4次重复实验 The fertilization for Lettuce,Pakchoi and tomato was based on recommended amount by Korean ru

31、ral Development Administration(RDA)Whole crops were tested as 3replcates(except Lettuce(n-4)1.1.作物作物 CropCrop 叶类蔬菜Leaf vegetable：生菜(Lettuce,Lactuca sativa),小白菜(Pak Choi,Brassica campestris L.ssp.Chinesis)果类蔬菜Fruit vegetable：西红柿(Tomato,Solanum lycopersicum var.cerasiforme)水果Fruit tree：苹果(Apple,Malus

32、domestica)2.2.实验实验日期日期 Year Year of trial of trial:2017,韩国（2017,in S.Korea）3.3.实验实验方案方案 Treatment Treatment：氨基酸Amino Acids：赖氨酸Lysine,苏氨酸Threonine,色氨酸Tryptophan,缬氨酸Valine,蛋氨酸Methionine,MSG,核苷酸IMP&GMP 作物实验实验 叶叶类类植物（生菜&小白菜）Crop test leaf vegetables（Lettuce&Pakchoi）干重干重Dry Dry weight(weight(l l叶叶片片eafea

33、f)(unit:g/plant)unit:g/plant)鲜鲜重重 Fresh Fresh weight weight(茎茎shoot)shoot)(unit:g/plant)unit:g/plant)叶叶面面积积Leaf Leaf area area (unit:cmunit:cm2 2/plant)/plant)干重干重Dry Dry weight weight(叶叶片片l leaf),eaf),(unit:g/plant)unit:g/plant)叶叶片片SPADSPAD值值 Leaf Leaf SPAD valueSPAD value (1)(1)生菜生菜 Lettuce Lettuc

34、e Effects of amino acids on growth of lettuce(25th Jul)叶叶面面积积Leaf Leaf area area (unit:cmunit:cm2 2/plant)/plant)Effects of amino acids on growth of pakchoi(25th Jul)叶叶片片SPADSPAD值值 Leaf Leaf SPAD valueSPAD value 鲜鲜重重Fresh Fresh weight weight(茎茎shoot)shoot)(unit:g/plant)unit:g/plant)(2)(2)小白菜小白菜 Pakc

35、hoiPakchoi A A B B D D F F C C GG E E A A B B D D F F C C GG E E A A B B D D F F C C GG E E A A B B D D F F C C GG E E A A B B D D F F C C GG E E A A B B D D F F C C GG E E A A B B D D F F C C GG E E A A B B D D F F C C GG E E GG HH HH HH HH HH HH HH HH Amino AcidsAmino Acids Amino AcidsAmino Acids

36、Amino AcidsAmino Acids Amino AcidsAmino Acids Amino AcidsAmino Acids Amino AcidsAmino Acids Amino AcidsAmino Acids Amino AcidsAmino Acids 提高提高叶叶类类菜的生菜的生长长和和产产量量 Improve growth and yield of leaf vegetables Improve growth and yield of leaf vegetables:H:H,A,A,B C,E&F B C,E&F 作物实验实验:果类类蔬菜（西红柿红柿）L-amino

37、acids Crop test Fruit vegetable(Tomato)可提高果可提高果实实重量及重量及数数量的量的氨氨基酸基酸 Improve fruit weight and quantity:H,A,C&F Improve fruit weight and quantity:H,A,C&F 可提高果可提高果实实含糖量的含糖量的氨氨基酸基酸 Improve Improve brix value of the fruitsbrix value of the fruits:H:H 含糖量 果实重量（g/株）果实数量（g/株）作物实验实验:果树树（苹苹果）L-amino acids Cro

38、p test Fruit Tree(Apple)可提高果可提高果实实重量重量氨氨基酸基酸 Improve fruit weight:H,A,C,D,E&F Improve fruit weight:H,A,C,D,E&F 可提高果可提高果实实含糖量的含糖量的氨氨基酸基酸 Improve brix value of the fruits:H&EImprove brix value of the fruits:H&E 均果重均果重 含糖量含糖量 进进一步计划计划 Further Plans 1 1 完成完成2020种单种单一一氨氨基酸基酸对对植物的作用模式分析植物的作用模式分析 Complete

39、identifying of mode of actions of 20 single Amino acidsComplete identifying of mode of actions of 20 single Amino acids 2 2 客客户户定制化配方定制化配方设计设计&功效功效实验实验 Customized Customized recipe design&Efficacy recipe design&Efficacy testtest 3 3 扩扩大大产产品范品范畴畴 Expanding product portfolioExpanding product portfolio

40、 配方配方设计设计 Design recipeDesign recipe 针对特定需求提供定制化配方设计 如抗旱、色泽等 Customized recipe design for specific purpose (e.g.drought,coloring etc.,)表型表型组学组学（温温室室实验实验 PHENOMICSPHENOMICS (Lab&Green hous(Lab&Green house e)对于温室实验作物使用氨基酸生物刺激剂的功效实验 Efficacy test of AA-biostimulant with target crop plants in the glass

41、house 大田大田实验实验 FIELD TRIALSFIELD TRIALS 对大田作物进行氨基酸生物刺激剂的功效实验 Efficacy test of AA-biostimulant with target crop plants in the field What is KIA?主要特征主要特征 Key characteristicsKey characteristics -100%环保原料生产组成 Made of 100%eco-friendly raw materials-对人体及环境无害（0残留）Harmless to human and environment(0 residue

42、)-液体 Liquid-pH:4.0 4.4-营养、保湿、耐用 Alimental,Moisturizing and Durability 由由氨氨基酸及有机酸基酸及有机酸组组成的天然肥料成的天然肥料 A natural fertilizer composed of Amino acids and Organic acidA natural fertilizer composed of Amino acids and Organic acid 产产品毒性品毒性 Product ToxicityProduct Toxicity 人人体体安全安全 Human Human SafetySafety

43、急性经口毒性Oral toxicity(LD50)氨基酸 Amino Acid 低于标准 Below standard(NLM:ChemDplus)有机酸 Organic Acid 低于标准 Below standard(OECD SIDS)皮肤刺激性/毒性 Skin irritation/toxicity 未检测出 Non-detection 环环境影境影响响Environmental Environmental ImpactImpact 微生物 Microorganism 未检出Non-detection 土壤污染Soil pollution 未检出Non-detection 生物降解性

44、Bio degradability 100%降解decomposed 对生态系统的危害 Toxicity to ecosystems 未检出Non-detection 功能功能 FunctionFunction Enhance Water holding capacityWater holding capacity 1 增强 持水能力 Interrupt wilt coefficient of sandy soil Extension of plant lifeExtension of plant life 2 阻断沙地植物 萎蔫系数,延长寿命 Strong interaction betwe

45、en KIA and nitrogen fertilizerKIA and nitrogen fertilizer 3 KIA及氮肥间 相互作用 Enhance Crop seeding germinationCrop seeding germination 4 促进作物 种子萌发 Precipitate Plant growth effectPlant growth effect 5 促进植物生长 CK450KIA1200KIA2400KIA900S450ACAboveground biomass(g/20cm)051015202530KIA 研研究报报告 KIA research repo

46、rt -中国科学院水利部水土保持研究所(Institute of Soil and Water Conservation)(2)(2)延延长长植物植物寿寿：玉米：玉米 Extend plant life:maizeExtend plant life:maize 实验方法实验方法Test method:Test method:对照CK(control KIA,no treat),丙烯酰胺Acrylamide(kg/公顷Ha),KIA(kg/KIA(kg/公顷公顷Ha)Ha)(1)(1)持水能力持水能力 Water holding capacityWater holding capacity(3)(3)促促进种进种苗生苗生长长:玉米玉米 Enhance seeding growth:maizeEnhance seeding growth:maize (4)(4)促促进进植物生植物生长长：黑：黑麦麦草草 Plant growth effect:ryegrassPlant growth effect:ryegrass